Our BIAcore T200 enables the label-free analysis of protein/protein as well as protein/small molecule interactions using a range of immobilisation strategies. This high performance platform can be used to understand molecular mechanisms and structure-function relationships, perform rapid and accurate calibration-free concentration analysis, define potential drug targets and screen and characterise drug candidates.The equipment is based in the Wellcome Trust Brenner Building and is a shared resource which implements a booking system and is accessible through the facility manager.
BioScreening Technology Group
Please visit the BioScreening Technology Group web pages here.
Clinical MRI facility
For information about the Clinical MRI facility, from the Multidisciplinary Cardiovascular Research Centre webpages, click here.
Digital pathology (whole slide imaging)
Established in 2003, the Leeds digital pathology project is one of the largest digital pathology projects in the world. Based at Wellcome Trust Brenner Building on St James's University Hospital site, it hosts four Aperio virtual slide scanners with a capacity of 2000-5000 slides per month. The system is available for viewing all virtual slides on the internet. Dedicated virtual slide storage includes 180Tb of online storage with local and remote backups.
Standard and large format histopathology slides can be scanned at 20x and 40x magnification, including whole sections, immunohistochemistry and tissue microarrays (TMAs). All slides are scanned by dedicated scanner technicians who perform a visual quality control of scan quality. In addition to standard vendor-supplied virtual slide serving and viewing software the project has in-house TMA software, stereometry software, custom image analysis development capacity and our unique 3D pathology software.
Our research interests are in clinical use of virtual slides including developing the novel "Leeds virtual microscope", a high performance digital pathology workstation, the "Powerwall", a wall sized display for teaching and collaboration, and image analysis.
The project has a dedicated website and web development capacity to design and host individual project websites. For further information and contact details click here
Health Flow Cytometry and Imaging Facility
Light Cytometry has developed significantly over the last ten years, in terms of the sampling sensitivity of equipment, sampling speed and the number of parameters that can be measured simultaneously. These advances in multi-dimensional data collection which can be taken on live material in real-time continues to place work using these technologies at the cutting edge of the biological sciences.
Our core facility aims to resource and deliver advanced light based cytometric equipment and cell purification technologies along with expert research support and training in these fields to expand the understanding of dynamic cellular functions both in vito and in vivo in a clinical setting.
The combined core facility has been expanding and developing in range of APPLICATIONS since its inception in 2007 and is well supported with a team of 2 expert senior research technicians and a facility manager with 17 years experience in the field.
Alongside the facility manager the facility is strategically led by it's two academic leads for the Flow Cytometry and Imaging arms of the combined facility and who are best contacted in person for potential collaborations.
The FoMH Core Flow Cytometry & Imaging Facility based within the Wellcome Trust Brenner Building and the Clinical Sciences Building on the St James's University Hospital Campus is well equipped to support range of research applications. The Facility is composed of 2 fluorescent activated cell sorters, 3 flow cytometer analysers, 1 metabolic analysers, 2 laser scanning confocal microscopes, 2 dedicated fluorescent live cell imaging platforms, 1 widefield fluorescent microscope, 1 high content-high throughput fluorescent plate microscope (with automated loader) and an array of specialist analysis software on our 5 dedicated online analysis platforms.
For further information please see:
The LIMM Histology service is based in the Wellcome Trust Brenner Building at St James's University Hospital. A full histology service is offered, including tissue fixation, processing into paraffin wax blocks, sectioning and staining. Two experienced staff provide the core service and the aim is to provide a maximum turnaround time of one week for routine specimens (processing and staining for haematoxylin and eosin). Urgent samples can be processed by special request.
For further information please visit the Virtual Pathology web pages.
Laser capture microscopy
The ArcturusXT™ Microdissection System combines both a gentle infrared (IR) laser ideal for capture of single cells and small numbers of cells, and Ultraviolet (UV) laser for precision cutting optimal for dense tissue structures and isolation of large numbers of cells and tissue areas. Captured material is suitable for various downstream processes that require nucleic acids or proteins, and expansion of captured live cell populations. This system is suitable for isolating material or cells from thick or thin tissue sections, frozen or fixed, stained or unstained sections, hydrated or dehydrated samples, fine needle aspirates and live cell cultures. The system is also equipped for fluorescence microscopy, this enables the user to identify and isolate target cells that have been fluorescently labelled.
Next Generation Sequencing
The NGS facility is a partnership between the University of Leeds & the Leeds Teaching Hospitals NHS Trust
The facility is currently equipped with Illumina HiSeq and MiSeq sequencers, and we can discuss which of these may meet your needs for sequencing of DNA or RNA samples. Depending on requirements, we can sequence user-prepared libraries or provide a limited library preparation service. The facility has a proven track record in the effective use of exome, RNASeq and Chip-Seq methodologies, and we are continuously striving to broaden our skill base.
As our team comprises members of the NHS diagnostic labs and University research staff, we are able to generate data with a full audit trail to NHS diagnostic standards while simultaneously proving a flexible environment that allows us to rapidly respond to new developments in your research field.
For further information please visit the NGS webpage
Please visit the Clinical and Biomedical Proteomics Group webpage
Research Tissue Banks
Leeds University is a founding member of the Breast Cancer Campaign Tissue Bank. For more information, please visit the webpage.
Currently the Real Time Facility comprises two work UV work stations (complete with designated Gilsons and multichannels) for PCR plate preparation, two ABI real time machines (7500 and a 7900HT), and a specialist centrifuge for loading Taqman Low Density Array (TLDA) plates. The ABI 7900HT has a 96 well and 384 well blocks, and the capacity to run TaqMan® low density micro-fluidic cards (TLDA). The 7500 can only run 96 well plates. The Facility supplies the plates/optical seals for both machines and tips (if required). Users are charged per run as a contribution to the service contracts and calibration kits.
GCLP Clinical Sample Processing
Please visit the Clinical and Biomedical Proteomics Group webpage
Disease Registry Datasets
Click here for more information on the following datasets:
- Yorkshire Specialist Register of Cancer in Children and Young People
- Yorkshire Register of Diabetes in Children and Young Adults
- Paediatric Intensive Care audit network (PICANet)
- Yorkshire Congenital Anomalies Register.
Data Management Group (DMG)
The Data Management Group (DMG) in the Leeds Institute of Cardiovascular and Metabolic Medicine, in conjunction with OneIT, operates the Secure Electronic Environment for Data (SEED) facility, which is a secure data management infrastructure. This allows for safe storage and access to patient-identifiable data, subject to individual consent or the relevant approval to set aside consent. The facility allows secure remote data entry and reporting over the web and a safe environment on-campus for record linkage, processing and statistical analysis. Data in the facility is clustered for resilience, backed-up nightly and can be encrypted if required.
The facility is currently used to underpin a range of biomedical and health research undertaken in the School of Medicine and more widely. DMG is managed by Mr Thomas Fleming MCITP and academic leadership is provided by Dr Paul Baxter.