Dr Anthony Buckley

Postdoctoral Research Fellow

0113 392 8663

Summary: My current research is on biofilm formation by Clostridium difficile, as a persistence mechanism during infection.

Location: Microbiology, Old Medical School, Leeds General Infirmary

Teaching Commitments: Primary supervisor to a Ph.D. student

Overview

My research is on biofilm formation by Clostridium difficile, as a persistence mechanism during infection. I use molecular techniques to understand the interaction of C. difficile with other gut bacteria and how they form a biofilm.

I started my Ph.D. investigating the role of efflux pumps in Salmonella Typhimurium multiple antimicrobial resistance mechanisms at the University of Birmingham. Following this, I determined the in vivo efficacy of vaccines against Campylobacter jejuni. By using different Salmonella Typhimurium strains expressing C. jejuni proteins I was able to determine the optimum window for vaccine delivery and showed effective vaccination to reduce C. jejuni levels entering the food chain. Since 2010, I have worked on the hospital-acquired bacterium Clostridium difficile, using molecular mechanisms to understand the in vivo pathogenesis of this organism and its interaction with the gut environment.