siRNA Screens

The BSTG has two siGenome smartpool whole genome siRNA libraries from Dharmacon, targeting mouse and human sequences. Each gene is targeted by a pool of four siRNAs in a single well. The siRNAs are introduced into cells where they reduce the expression of a specific gene.

The BSTG will;

  • help optimise transfection efficiencies and confirm knockdown of control genes prior to any further work.
  • screen whole genome libraries or specific pre-defined partial-libraries.  Pre-defined libraries specifically knockdown gene families or genes that have related functions.

Sub-libraries are listed below (*not available as sub-libraries unless they are bought directly from Dharmacon).

siGENOME human libraries image. BHRC

siGENOME Mouse Libraries BHRC